Dag Assay Kit Cell Biolabs

Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Description: Our Lactate Assay Kit measures L-lactate in biological samples. Lactate is first oxidized by lactate oxidase, yielding pyruvate and hydrogen peroxide. The hydrogen peroxide released from this reaction is specifically detected by either a colorimetric or fluorometric probe in a 1:1 ratio. Lactate levels in unknown samples are determined based on a lactate standard curve.

Description: Our Lactate Assay Kit measures L-lactate in biological samples. Lactate is first oxidized by lactate oxidase, yielding pyruvate and hydrogen peroxide. The hydrogen peroxide released from this reaction is specifically detected by either a colorimetric or fluorometric probe in a 1:1 ratio. Lactate levels in unknown samples are determined based on a lactate standard curve.

Description: Our NAD+/NADH Assay Kit detects NAD+ and NADH in cell and tissue lysates. Total NAD+/NADH can be detected or samples can be treated with an acid or base treatment to specifically detect NAD+ or NADH. This assay uses an enzymatic cycling reaction that reduces NAD+ to NADH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NAD+/NADH levels in unknown samples are calculated based on the provided NAD+ standard curve.

Description: Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.

Description: Our BCG Albumin Assay utilizes a proprietary bromocresol green (BCG) formulation for the detection of albumin in biological samples. BCG forms a color complex after 5 minutes when bound to albumin and is detected colorimetrically between 570-670nm. The amount of albumin in samples is determined based on a provided albumin standard curve.

Description: Our NADP+/NADPH Assay Kit detects NADP+ and NADPH in cell and tissue lysates. Total NADP+/NADPH can be detected or samples can be treated with an acid or base treatment to specifically detect NADP+ or NADPH. This assay uses an enzymatic cycling reaction that reduces NADP+ to NADPH, which then reacts with a colorimetric probe and is detected with a spectrophotometric plate reader at 450nm. NADP+/ NADPH levels in unknown samples are calculated based on the provided NADP+ standard curve.

Description: Our Glycogen Assay Kit (Fluorometric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-570 nm/em. 590-600 nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.

Description: Our Glycogen Assay Kit (Colorimetric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve.

Description: Our Phosphatidylglycerol/Cardiolipin Assay Kit measures both phosphatidylglycerol and cardiolipin in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidylglycerol and cardiolipin to glycerol, which is then phosphorlyated by glycerol kinase to yield glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidylglycerol and cardiolipin levels in unknown sampled are determined based on the provided cardiolipin standard curve.

Description: Our Starch Assay Kit (Colorimetric) measures starch in food samples. First, starch is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Starch levels in unknown samples are determined based on the provided starch standard curve.

Description: Our Starch Assay Kit (Fluorometric) measures starch in food samples. First, starch is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-570 nm/em. 590-600 nm. Starch levels in unknown samples are determined based on the provided starch standard curve.

Description: Our Homocitrulline/Citrulline Assay Kit detects total homocitrulline/citrulline in plasma, serum, urine, or cell and tissue lysates. Samples are first treated with sodium dodecyl sulfate (SDS) and proteinase K to release free homocitrulline/citrulline residues. Assay reagents are then added, which react with homocitrulline and citrulline to produce a chromophore. The absorbance is read at 540-560nm and the levels of homocitrulline/citrulline are determined based on the provided standard curve.

Description: Cell Biolabs? Choline Assay Kits are simple assays that measure the amount of choline present in a variety of sample types in a convenient 96-well microtiter plate format.  Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples.  Sample choline concentrations are determined by comparison with a known choline standard and measured on a fluorescence plate reader.

Description: Cell Biolabs? Choline Assay Kits are simple assays that measure the amount of choline present in a variety of sample types in a convenient 96-well microtiter plate format.  Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples.  Sample choline concentrations are determined by comparison with a known choline standard and measured on a colorimetric plate reader.

Description: Cell Biolabs? Creatinine Assay Kit measures creatinine levels in urine. Samples are compared to a known concentration of creatinine standard within a 96-well microtiter plate format. Samples and standards are incubated for 30 minutes with a reaction reagent which changes color from yellow to bright orange upon reacting with creatinine, forming the creatinine-picrate complex. The plate is read with a standard 96-well spectrophotometric microplate reader at 490 nm. Higher OD values correlate with high creatinine concentrations. Sample creatinine concentrations are determined by comparison with the known creatinine standards.