Mmp-1 Assay Kit Enzo

Description: Minimum order quantity: 1 unit of 150TST

Description: Minimum order quantity: 1 unit of 150AS

Description: Minimum order quantity: 1 unit of 150AS

Description: The TNKS1 Chemiluminescent Assay Kit is designed to measure the activity of Tankyrase 1 (TNKS1, also known as PARP5A) for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS1 (PARP5A) Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS1 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The TNKS2 Chemiluminescent Assay Kit is designed to measure the activity of Tankyrase 2 (TNKS2, also known as PARP5B) for screening and profiling applications. TNKS2 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS2 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS2 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

Description: The Kynurenine 3-Monooxygenase (KMO) Inhibitor Screening Assay Kit is designed to measure human KMO enzyme inhibition. The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions. The KMO Inhibitor Screening Assay Kit is simple to use. Recombinant hKMO is mixed with inhibitors and the reaction is initiated through addition of the substrates NADPH and L-Kynurenine. After a room temperature incubation, activity is determined by measuring the absorption of the reaction product at λ=412 nm. The UV absorption signal correlates with the amount of NADPH remaining in the reaction.

Description: The JMJD2C Homogeneous Assay Kit is designed to measure JMJD2C activity for screening and profiling applications. JMJD2C is a JumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylation activity toward H3-K₉Me³ and H3-K₃₆Me³. The JMJD2C Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JMJD2C for 96 enzyme reactions. The key to the JMJD2C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JMJD3 Homogeneous Assay Kit is designed to measure JMJD3 activity for screening and profiling applications. JMJD3, also known as KDM6B, is a JumonjiC (JmjC) domain protein that exhibits demethylation activity toward H3-K₂₇Me³ and H3-K₂₇Me². The key to the JMJD3 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD3 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JARID1A Homogeneous Assay Kit is designed to measure JARID1A activity for screening and profiling applications. JARID1A, also known as RBBP2 and KDM5A, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K₄Me² and H3-K₄Me³. The JARID1A Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1A for 96 or 384 enzyme reactions. The key to the JARID1A Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1A enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated histone substrate. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JARID1C Homogeneous Assay Kit is designed to measure JARID1C activity for screening and profiling applications. JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K₄Me² and H3-K₄Me³. The JARID1C Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1C for 96 enzyme reactions. The key to the JARID1C Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1C enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.Our histone demethylase assays utilize highly specific antibodies that recognize demethylated histone substrate. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The JARID1B Homogeneous Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K₄Me² and H3-K₄Me³. The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA^® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The PHF8 Homogeneous Assay Kit is designed to measure PHF8activity for screening and profiling applications. PHF8, also known as JHDM1F and KDM7B, is aJumonjiC (JmjC) domain containing histone lysine demethylase that exhibits demethylationactivity toward H3-K9Me2. The PHF8 Homogeneous Assay Kit comes in a convenientAlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primaryantibody, demethylase assay buffer, and purified PHF8 for 96 enzyme reactions. The key to thePHF8 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylatedsubstrate. With this kit, only three simple steps on a microtiter plate are required formethyltransferase detection. First, a sample containing PHF8 enzyme is incubated with thebiotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads,followed by reading the Alpha-counts._x000D_

Description: The FBXL10 (KDM2B) Homogeneous Assay Kit is designed to measure the activity of the FBXL10 for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, and CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyl-lysine 4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, a sample containing FBXL10 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Description: The FBXL11 Homogeneous Assay Kit is designed to measure FBXL11
activity for screening and profiling applications. FBXL11, also known as KDM2A, JHDM1A, and Ndy2, is a histone lysine demethylase that exhibits demethylation activity toward H3-K₃₆Me².The key to the FBXL11 Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing FBXL11 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts._x000D_