H6008-95G Elisa Kit Bioassay

Description: A competitive ELISA for quantitative measurement of Mouse True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Mouse True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rabbit True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rat True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: A competitive ELISA for quantitative measurement of Rat True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Description: An accurate, simple, fast (15 min) and inexpensive screening tool for the identification of protein putrefaction in the gastrointestinal tract. For research use only, not intended for diagnostic use. The Indican Reagent is corrosive. It is recommended to perform the test in a chemical fume hood. Wear gloves, goggles and protective clothing. Key Features: Convenient. Only need to pipette 2 mL urine into the ready reagent vial, mix and read the indican level from a color chart. Fast: 15 min. Method: Obermeyer (Improved). Samples: Urine. Species: Human. Procedure: Assay takes 15 min. Kit size: 20 tests.

Description: For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis. Key Features: Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure take 20 min. Robust and amenable to HTS. Single reagent, "mix-incubate-measure" type assay. High-throughput assay in 96-well plates allows simultaneous processing tens of thousands of samples per day. Method: OD500nm. Samples: Cell culture. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests.

Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

Description: For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng.

Description: Quantitative determination of sucrose by colorimetric (565nm) method. Procedure: 70 min. Kit size: 100 tests. Detection limit: 17 µM. Shelf life: 3 months. Shipping: on ice; storage: -20°C.

Description: Quantitative determination of fructose by colorimetric (565nm) method. Procedure: 70 min. Kit size: 100 tests. Detection limit: 12 µM fructose. Shelf life: 3 months. Shipping: on ice; storage: -20°C.

Description: Quantitative determination of lactulose by colorimetric (565nm) method. Procedure: 60 min. Kit size: 100 tests. Detection limit: 3 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

Description: For quantitative determination of myeloperoxidase enzyme activity and evaluation of drug modulators. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.0025 to 2 U/L for 10 min reaction at 25°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Cell lysates, tissues, etc. Species: All. Procedure: Assay takes approximately 30 min. Kit size: 100 tests. Detection limit: 0.0025 U/L.

Description: For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

Description: For rapid, quantitative, bioluminescent determination of ATP and evaluation of drug effects on ATP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ATP or a single cell can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of > 0.5 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 0.02 µM.

Description: For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 µM.

Description: For quantitative determination of ADP determination in cells and other biological samples. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/590nm. Samples: Cells and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.1 µM.

Description: For quantitative determination of arginase activity and screen for its inhibitors. Key Features: Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format. Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD430nm. Samples: Enzyme preparations, serum, plasma, tissue culture etc. Species: All. Procedure: Assay takes 2 hrs. Kit size: 100 tests. Detection limit: 1 U/L (0.3 U/L for 2 hr reaction).

Description: For quantitative determination of DNA. Key Features: Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day. Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01% SDS) and common DNA extraction buffer do not interfere in the assay. Method: FL350/450nm. Samples: Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 100 ng/mL.

Description: For quantitative determination of ferric reduction antioxidant potential (FRAP). Key Features: Sensitive and accurate. Linear detection range: 0.5 to 180 µM Fe³⁺ reduction potential in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Method: OD590nm. Samples: Plant extracts, foods, vitamins, supplements, and biological samples such as serum, plasma, and urine. Species: All. Procedure: Assay takes 40 min. Kit size: 250 tests. Detection limit: 0.5 µM.

Description: For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism. Key Features: Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Biological (e.g. serum) and environmental samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 27 µg/dL (4.8 µM).

Description: For quantitative determination of heme and evaluation of drug effects on heme metabolism. Key Features: Sensitive and accurate. Linear detection range 0.6 - 125 µM heme in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents are non-toxic. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.6 µM.