Succinate Assay Kit Bioassay Systems

Description: Human Anti-Salmonella IgG Antibody Assay Kit

Description: Mouse Anti-SEB IgG Antibody Assay Kit

Description: Mouse Anti-SEA IgG Antibody Assay Kit

Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Description: For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time 15 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Malate in food, juice, beverage and other agricultural products. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.02 mM.

Description: For sensitive and high-throughput phosphate determination. Key Features: Reagent very stable. Due to our innovative formulation, no precipitation of reagent occurs. Therefore no filtration of reagent is needed prior to assays, as is often required with other commercial kits. High sensitivity and wide detection range: detection of as little of 20 pmoles of phosphate and useful range between 0.4 µM and 50 µM phosphate. Fast and convenient: single reagent "mix-and-measure" assay allows quantitation of free phosphate within 30 minutes. Compatible with routine laboratory and HTS formats: assays can be performed in tubes, cuvettes or microplates, on spectrophotometers and plate readers. Robust and amenable to HTS: Z factors of 0.7 to 0.9 are observed in 96-well plates. Can be readily automated on HTS liquid handling systems Method: OD620nm (malachite green). Samples: Enzyme reactions with free phosphate releases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 0.4 µM.

Description: Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.

Description: Sensitive determination of NADP and NADPH by fluorometric (530/585nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.01 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

Description: For quantitative determination of starch and evaluation of drug effects on starch metabolism. Key Features: Use as little as 10 µL samples. Linear detection range: 2 to 200 µg/mL starch for colorimetric assays and 0.2 to 20 µg/mL for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Biological, agriculture, food etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

Description: For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.

Description: For quantitative determination of lipid peroxides (thiobarbituric acid reactive substances, TBARS) and evaluation of drug effects on lipid peroxidation. Key Features: Sensitive and accurate. Linear detection range: colorimetric assay 1 - 30 µM, fluorometric assay 0.1 - 1.5 µM MDA. Method: OD535nm, or FL530/550nm. Samples: Serum, plasma, urine, saliva etc. Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 0.1 µM.

Description: For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism. Key Features: High sensitivity and wide linear range. Use 20 µL sample. Linear detection range 24 to 1000 µM ammonia. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ammonia within 30 minutes. Method: OD340nm, or FL360/450nm. Samples: Serum, plasma, urine, saliva, cell culture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 24 µM.

Description: For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. Key Features: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature and read. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, food, agriculture and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.13 mM.

Description: For quantitative determination of oxalate (oxalic acid) in urine and other samples. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10 minute incubation: 20 to 1500 µM oxalate. Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor’s assay kits. High-throughput adaptable. The procedure involves addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day. Method: OD595nm. Samples: urine, animal and plant tissue samples. Species: All. Procedure: Assay takes 10 min. Kit size: 100 tests. Detection limit: 20 µM.

Description: For quantitative determination of maltose and evaluation of drug effects on its metabolism. Key Features: Fast and sensitive. Use of 10 µL sample. Linear detection range 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for flourimetric assays. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Serum, urine, food and beverages, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 2 µM.

Description: For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 µM.

Description: For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. Key Features: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 5 to 300 µM (90 µg/dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 µM for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Method: OD570nm, or FL530/585nm. Samples: Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 5 µM.

Description: For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism. Key Features: Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM L-Lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM L-Lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Serum, plasma, cell culture media etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.05 mM.

Description: Quantitative determination of NADP and NADPH by colorimetric (565nm) method. Procedure: 30 min. Kit size: 100 tests. Detection limit: 0.1 µM. Shelf life: 6 months. Shipping: on ice; storage: -20°C.

Description: For quantitatve determination of collagen in biological and cosmetic products. Key Features: Safe. Non-radioactive assay. Fast and sensitive. Linear detection range (20µL sample): 2 µg/mL to 50µg/mLcollagen in 96-well plate assay. Convenient and high-throughput."Add-mix-read" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: FL375/465nm. Samples: Biological samples (e.g. plasma, serum, cells) and cosmetic samples (e.g. collagen solution). Species: All. Procedure: Assay takes 80 min. Kit size: 100 tests. Detection limit: 2 µg/mL.

Description: For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. Key Features: Sensitive and accurate. Detection limit 0.0008 vol % (140 µM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay. Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm (Enzymatic). Samples: Serum, plasma, urine, saliva samples etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.0008%.