Tick-borne nairoviruses (order Bunyavirales) encode an ovarian tumor area protease (OTU) that suppresses the innate immune response by reversing the post-translational modification of proteins by ubiquitin (Ub) and interferon-stimulated gene product 15 (ISG15). Ub is extremely conserved throughout eukaryotes, whereas ISG15 is simply current in vertebrates and reveals substantial sequence variety. Prior makes an attempt to handle the impact of ISG15 variety on viral protein-ISG15 interactions have centered on solely a single species’ ISG15 or a restricted choice of nairovirus OTUs.
To achieve a extra full perspective of OTU-ISG15 interactions, we biochemically assessed the relative actions of 14 various nairovirus OTUs for 12 species’ ISG15 and located that ISG15 exercise is predominantly restricted to specific nairovirus lineages reflecting, on the whole, recognized virus-host associations. To uncover the underlying molecular elements driving OTUs affinity for ISG15, X-ray crystal constructions of Kupe virus and Ganjam virus OTUs sure to sheep ISG15 had been solved and in comparison with complexes of Crimean-Congo hemorrhagic fever virus and Erve virus OTUs sure to human and mouse ISG15, respectively. By means of mutational and structural evaluation seven residues in ISG15 had been recognized that predominantly affect ISG15 species specificity amongst nairovirus OTUs.
Moreover, OTU residues had been recognized that affect ISG15 choice, suggesting the potential for viral OTUs to adapt to completely different host ISG15s. These findings present a basis to additional develop analysis strategies to hint nairovirus-host relationships and delineate the total affect of ISG15 variety on nairovirus an infection.
Substrate-to-Product Conversion Facilitates Energetic Web site Loop Opening in Yeast Enolase: A Molecular Dynamics Examine.
Yeast enolase serves as a prototype for metalloenzymes with labile, catalytic lively website steel ions and is vital for glycolysis and fermentation processes. Herein, microsecond molecular dynamics simulations of the protein-substrate and protein-product complexes are performed to elucidate the mechanism of the opening of catalytically vital lively website loops. These simulations point out that conversion of substrate to product is accompanied by diminished steel coordination and hydrogen-bonding interactions, in addition to enhanced loop flexibility.
Furthermore, free vitality simulations present that the loop opening is endergonic when substrate is sure however exergonic when product is sure. Thus, the conversion to product weakens the affiliation of the loop with the ligand and binding website, thereby facilitating the loop opening after catalysis and enabling product launch. These insights about lively website loop motions in enzyme catalysis could also be helpful in guiding enzyme design efforts. The triterpenes are structurally various group of specialised metabolites with vital roles in plant protection and human well being. Glycyrrhizin, with a carboxyl group at C-30 of its aglycone moiety, is a beneficial triterpene glycoside, the manufacturing of which is restricted to legume medicinal vegetation belonging to the Glycyrrhiza species.
Cytochrome P450 monooxygenases (P450s) are vital for producing triterpene chemodiversity by catalyzing site-specific oxidation of the triterpene scaffold. CYP72A154 was beforehand recognized from the glycyrrhizin-producing plant Glycyrrhiza uralensis as a C-30 oxidase in glycyrrhizin biosynthesis, however its regioselectivity is slightly low. In distinction, CYP72A63 from Medicago truncatula confirmed superior regioselectivity in C-30 oxidation, enhancing the manufacturing of glycyrrhizin aglycone in engineered yeast. The underlying molecular foundation of C-30 product regioselectivity is just not properly understood.
Molecular recognition within the product website of cellobiohydrolase Cel7A regulates processive step size.
Cellobiohydrolase Cel7A is an industrial vital enzyme that breaks down cellulose by a fancy processive mechanism. The enzyme threads the lowering finish of a cellulose strand into its tunnel-shaped catalytic area and progresses alongside the strand whereas sequentially releasing the disaccharide cellobiose. Whereas some molecular particulars of this intricate course of have emerged, normal structure-function relationships for Cel7A stay poorly elucidated. One attention-grabbing side is the prevalence of notably sturdy ligand interactions within the product binding website. On this work, we analyze these interactions in Cel7A from Trichoderma reesei with particular emphasis on the Arg251 and Arg394 residues.
We made intensive biochemical characterization of enzymes that had been mutated in these two positions and confirmed that the arginine residues contributed strongly to product binding. Particularly, about 50% of the full normal free vitality of product binding may very well be ascribed to 4 hydrogen bonds to Arg251 and Arg394, which had beforehand been recognized in crystal constructions. Mutation of both Arg251 or Arg394 lowered manufacturing inhibition of Cel7A, however on the similar time altered the enzyme product profile and resulted in about 50% discount in each processivity and hydrolytic exercise.
BCIP (Molecular Biology Grade) |
CE108 |
GeneOn |
250 mg |
EUR 63 |
BCIP (Molecular Biology Grade) |
CE109 |
GeneOn |
1 g |
EUR 90 |
CHAPS (Molecular Biology Grade) |
CE114 |
GeneOn |
1 g |
EUR 55 |
CHAPS (Molecular Biology Grade) |
CE115 |
GeneOn |
5 g |
EUR 131 |
CHAPS (Molecular Biology Grade) |
CE116 |
GeneOn |
25 g |
EUR 410 |
DAPI (Molecular Biology Grade) |
CE117 |
GeneOn |
5 mg |
EUR 60 |
DAPI (Molecular Biology Grade) |
CE118 |
GeneOn |
25 mg |
EUR 133 |
DAPI (Molecular Biology Grade) |
CE119 |
GeneOn |
100 mg |
EUR 319 |
Dimethylsulfoxide (Molecular Biology Grade) |
CE120 |
GeneOn |
100 ml |
EUR 55 |
Dimethylsulfoxide (Molecular Biology Grade) |
CE121 |
GeneOn |
500 ml |
EUR 92 |
DTT (Molecular Biology Grade) |
CE131 |
GeneOn |
5 g |
EUR 78 |
DTT (Molecular Biology Grade) |
CE132 |
GeneOn |
10 g |
EUR 111 |
DTT (Molecular Biology Grade) |
CE133 |
GeneOn |
25 g |
EUR 203 |
Glycine (Molecular Biology Grade) |
CE158 |
GeneOn |
1 kg |
EUR 70 |
Glycine (Molecular Biology Grade) |
CE159 |
GeneOn |
5 kg |
EUR 190 |
HEPES (Molecular Biology Grade) |
CE171 |
GeneOn |
100 g |
EUR 82 |
HEPES (Molecular Biology Grade) |
CE172 |
GeneOn |
500 g |
EUR 224 |
HEPES (Molecular Biology Grade) |
CE173 |
GeneOn |
1 kg |
EUR 354 |
Lysozyme (Molecular Biology Grade) |
CE188 |
GeneOn |
1 g |
EUR 59 |
Lysozyme (Molecular Biology Grade) |
CE189 |
GeneOn |
10 g |
EUR 206 |
NAD (Molecular Biology Grade) |
CE196 |
GeneOn |
1 g |
EUR 60 |
NAD (Molecular Biology Grade) |
CE197 |
GeneOn |
5 g |
EUR 138 |
NBT (Molecular Biology Grade) |
CE209 |
GeneOn |
1 g |
EUR 103 |
NBT (Molecular Biology Grade) |
CE210 |
GeneOn |
5 g |
EUR 300 |
Tris (Molecular Biology Grade) |
CE237 |
GeneOn |
500 g |
EUR 89 |
Tris (Molecular Biology Grade) |
CE238 |
GeneOn |
1 kg |
EUR 128 |
Tris (Molecular Biology Grade) |
CE239 |
GeneOn |
5 kg |
EUR 446 |
Tween20 (Molecular Biology Grade) |
CE242 |
GeneOn |
1 l |
EUR 89 |
Water (Molecular Biology Grade) |
CE243 |
GeneOn |
500 ml |
EUR 52 |
Water (Molecular Biology Grade) |
CE244 |
GeneOn |
1 l |
EUR 56 |
Water, Ultrapure Molecular Biology Grade |
41024-4L |
Biotium |
4L |
EUR 121 |
Description: Minimum order quantity: 1 unit of 4L |
Ammonium sulfate (Molecular Biology Grade) |
CE105 |
GeneOn |
250 g |
EUR 46 |
Ammonium sulfate (Molecular Biology Grade) |
CE106 |
GeneOn |
1 kg |
EUR 60 |
Ammonium sulfate (Molecular Biology Grade) |
CE107 |
GeneOn |
5 kg |
EUR 128 |
Bis-Acrylamid (Molecular Biology Grade) |
CE110 |
GeneOn |
50 g |
EUR 79 |
Bis-Acrylamid (Molecular Biology Grade) |
CE111 |
GeneOn |
250 g |
EUR 216 |
Formamide deionized (Molecular Biology Grade) |
CE145 |
GeneOn |
500 ml |
EUR 73 |
Formamide deionized (Molecular Biology Grade) |
CE146 |
GeneOn |
1 l |
EUR 100 |
Glycerol 87 % (Molecular Biology Grade) |
CE154 |
GeneOn |
1 l |
EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
CE155 |
GeneOn |
500 ml |
EUR 65 |
Glycerol waterfree (Molecular Biology Grade) |
CE156 |
GeneOn |
1 l |
EUR 85 |
Glycerol waterfree (Molecular Biology Grade) |
CE157 |
GeneOn |
2.5 l |
EUR 142 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
CE160 |
GeneOn |
100 g |
EUR 78 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
CE161 |
GeneOn |
250 g |
EUR 128 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
CE162 |
GeneOn |
500 g |
EUR 194 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
CE163 |
GeneOn |
1 kg |
EUR 294 |
Guanidine Thiocyanate (Molecular Biology Grade) |
CE164 |
GeneOn |
100 g |
EUR 72 |
Guanidine Thiocyanate (Molecular Biology Grade) |
CE165 |
GeneOn |
500 g |
EUR 160 |
Guanidine Thiocyanate (Molecular Biology Grade) |
CE166 |
GeneOn |
1 kg |
EUR 256 |
MOPS buffer (Molecular Biology Grade) |
CE194 |
GeneOn |
100 g |
EUR 85 |
MOPS buffer (Molecular Biology Grade) |
CE195 |
GeneOn |
250 g |
EUR 141 |
Sodium chloride (Molecular Biology Grade) |
CE205 |
GeneOn |
500 g |
EUR 52 |
Sodium chloride (Molecular Biology Grade) |
CE206 |
GeneOn |
1 kg |
EUR 59 |
Sodium chloride (Molecular Biology Grade) |
CE207 |
GeneOn |
5 kg |
EUR 103 |
D(+)-Sucrose (Molecular Biology Grade) |
CE224 |
GeneOn |
500 g |
EUR 56 |
D(+)-Sucrose (Molecular Biology Grade) |
CE225 |
GeneOn |
1 kg |
EUR 70 |
D(+)-Sucrose (Molecular Biology Grade) |
CE226 |
GeneOn |
5 kg |
EUR 173 |
Tris - Hydrochloride (Molecular Biology Grade) |
CE234 |
GeneOn |
250 g |
EUR 83 |
Tris - Hydrochloride (Molecular Biology Grade) |
CE235 |
GeneOn |
500 g |
EUR 120 |
Tris - Hydrochloride (Molecular Biology Grade) |
CE236 |
GeneOn |
1 kg |
EUR 186 |
TritonX-100 (Molecular Biology Grade) |
CE240 |
GeneOn |
500 ml |
EUR 56 |
TritonX-100 (Molecular Biology Grade) |
CE241 |
GeneOn |
1 l |
EUR 66 |
Tween 20, Molecular Biology Grade |
T9100-010 |
GenDepot |
100ml |
EUR 72 |
Tween 20, Molecular Biology Grade |
T9100-050 |
GenDepot |
500ml |
EUR 111 |
Tween 20, Molecular Biology Grade |
T9100-100 |
GenDepot |
1L |
EUR 134 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
CE135 |
GeneOn |
250 g |
EUR 60 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
CE136 |
GeneOn |
500 g |
EUR 72 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
CE137 |
GeneOn |
1 kg |
EUR 104 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
CE138 |
GeneOn |
5 kg |
EUR 349 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
CE148 |
GeneOn |
500 g |
EUR 56 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
CE149 |
GeneOn |
1 kg |
EUR 63 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
CE150 |
GeneOn |
5 kg |
EUR 150 |
Yeast extract powder (Molecular Biology Grade) |
CE169 |
GeneOn |
500 g |
EUR 111 |
Hyaluronidase Grade I (Molecular Biology Grade) |
CE174 |
GeneOn |
1 g |
EUR 194 |
Hyaluronidase Grade I (Molecular Biology Grade) |
CE175 |
GeneOn |
5 g |
EUR 767 |
Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
CE190 |
GeneOn |
500 g |
EUR 82 |
NADH - Disodium salt (Molecular Biology Grade) |
CE198 |
GeneOn |
1 g |
EUR 76 |
NADH - Disodium salt (Molecular Biology Grade) |
CE199 |
GeneOn |
5 g |
EUR 204 |
NADP - sodium salt (Molecular Biology Grade) |
CE200 |
GeneOn |
250 mg |
EUR 77 |
NADP - sodium salt (Molecular Biology Grade) |
CE201 |
GeneOn |
1 g |
EUR 159 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
CE202 |
GeneOn |
25 mg |
EUR 59 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
CE203 |
GeneOn |
100 mg |
EUR 105 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
CE204 |
GeneOn |
500 mg |
EUR 312 |
SSC Buffer (20X) (Molecular Biology Grade) |
CE229 |
GeneOn |
1 l |
EUR 72 |
XTT sodium salt (Molecular Biology Grade) |
CE250 |
GeneOn |
100 mg |
EUR 174 |
XTT sodium salt (Molecular Biology Grade) |
CE251 |
GeneOn |
500 mg |
EUR 510 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
CE100 |
GeneOn |
50 g |
EUR 107 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
CE101 |
GeneOn |
100 g |
EUR 161 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
CE102 |
GeneOn |
250 g |
EUR 323 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
CE103 |
GeneOn |
500 g |
EUR 547 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
CE104 |
GeneOn |
1 kg |
EUR 969 |
Agarose LE, Ultra-Pure Molecular Biology Grade, 100 g |
41028-100G |
Biotium |
100G |
EUR 222 |
Description: Minimum order quantity: 1 unit of 100G |
Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g |
41028-25G |
Biotium |
25G |
EUR 109 |
Description: Minimum order quantity: 1 unit of 25G |
Agarose LE, Ultra-Pure Molecular Biology Grade, 500 g |
41028-500G |
Biotium |
500G |
EUR 719 |
Description: Minimum order quantity: 1 unit of 500G |
EDTA solution pH 8.0 (0.5 M) (Molecular Biology Grade) |
CE141 |
GeneOn |
500 ml |
EUR 73 |
LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
CE178 |
GeneOn |
500 g |
EUR 91 |
LB-Agar - Powder according to Lennox (Molecular Biology Grade) |
CE179 |
GeneOn |
2.5 kg |
EUR 251 |
LB-Agar - Powder according to Miller (Molecular Biology Grade) |
CE180 |
GeneOn |
500 g |
EUR 87 |
LB-Agar - Powder according to Miller (Molecular Biology Grade) |
CE181 |
GeneOn |
2.5 kg |
EUR 246 |
LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
CE182 |
GeneOn |
500 g |
EUR 90 |
LB-Medium - Powder according to Lennox (Molecular Biology Grade) |
CE183 |
GeneOn |
2.5 kg |
EUR 251 |
LB-Medium - Powder according to Miller (Molecular Biology Grade) |
CE184 |
GeneOn |
2.5 kg |
EUR 246 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT |
AGR-LE-100 |
CORNING |
1/pk |
EUR 168 |
Description: Bioscience Mol Bio; Agarose |
100 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
46-000-CI |
CORNING |
100 mL/pk |
EUR 57 |
Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
1 L, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
46-000-CM |
CORNING |
1L/pk |
EUR 98 |
Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
500 ML, MOLECULAR BIOLOGY GRADE WATER; TESTED TO USP STERILE PURIFIED WATER SPECIFICATIONS |
46-000-CV |
CORNING |
500 mL/pk |
EUR 106 |
Description: Media Catalog; Sterile Wi-Fi Qual, Cell |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE |
AGR-LE-5001 |
CORNING |
1/pk |
EUR 3058 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE MS, PCR SCREENING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE |
AGR-MS-1001 |
CORNING |
1/pk |
EUR 2038 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE |
AGR-LE-1001 |
CORNING |
1/pk |
EUR 772 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL) |
AGR-LM-1001 |
CORNING |
1/pk |
EUR 3737 |
Description: Bioscience Mol Bio; Agarose |
AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL) |
AGR-LM-501 |
CORNING |
1/pk |
EUR 2075 |
Description: Bioscience Mol Bio; Agarose |
Water Phenol |
abx082109-250ml |
Abbexa |
250 ml |
EUR 189 |
|
ELISA kit for Rat PST (Phenol Sulfotransferase) |
ELK6740 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Human PST (Phenol Sulfotransferase) |
ELK3246 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
ELISA kit for Mouse PST (Phenol Sulfotransferase) |
ELK3642 |
ELK Biotech |
1 plate of 96 wells |
EUR 432 |
|
Description: A sandwich ELISA kit for detection of Phenol Sulfotransferase from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. |
3-(Aminomethyl)phenol |
20-abx185751 |
Abbexa |
-
EUR 425.00
-
EUR 2277.00
-
EUR 857.00
|
|
|
Phenol, 4-propoxy- |
20-abx184303 |
Abbexa |
|
|
|
2-(Aminomethyl)phenol |
20-abx185699 |
Abbexa |
-
EUR 314.00
-
EUR 1442.00
-
EUR 592.00
|
|
|
Phenol Red Solution |
CA013-010 |
GenDepot |
100ml |
EUR 69 |
Phenol, saturated (pH7.9) |
PC6910 |
Bio Basic |
100ml |
EUR 76.1 |
|
Phenol, saturated (pH4.5) |
PC6920 |
Bio Basic |
100ml |
EUR 76.1 |
|
30UL CLEAR PIPET TIPS FOR MOLECULAR DEVICE LIBERTY. |
LT-384-R |
CORNING |
3840/pk |
EUR 2193 |
Description: Robotic Tips; Robotic tips - Axygen |
Molecular Weight Marker |
abx098957-15ul |
Abbexa |
15 ul |
EUR 105 |
|
Agarose, Molecular Grade |
BIO-41025 |
Bioline |
500g |
Ask for price |
Agarose, Molecular Grade |
BIO-41026 |
Bioline |
100g |
Ask for price |
Recombinant Phenol Sulfotransferase (PST) |
4-RPB268Hu01 |
Cloud-Clone |
-
EUR 481.70
-
EUR 232.00
-
EUR 1531.36
-
EUR 577.12
-
EUR 1054.24
-
EUR 385.00
-
EUR 3678.40
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Human Phenol Sulfotransferase expressed in: E.coli |
Recombinant Phenol Sulfotransferase (PST) |
4-RPB268Mu01 |
Cloud-Clone |
-
EUR 490.66
-
EUR 234.00
-
EUR 1564.96
-
EUR 588.32
-
EUR 1076.64
-
EUR 391.00
-
EUR 3762.40
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Mouse Phenol Sulfotransferase expressed in: E.coli |
Recombinant Phenol Sulfotransferase (PST) |
4-RPB268Ra01 |
Cloud-Clone |
-
EUR 510.37
-
EUR 239.00
-
EUR 1638.88
-
EUR 612.96
-
EUR 1125.92
-
EUR 404.00
-
EUR 3947.20
|
-
100 ug
-
10ug
-
1 mg
-
200 ug
-
500 ug
-
50ug
-
5 mg
|
|
Description: Recombinant Rat Phenol Sulfotransferase expressed in: E.coli |
Phenol Sulfotransferase (PST) Antibody |
20-abx129668 |
Abbexa |
-
EUR 425.00
-
EUR 133.00
-
EUR 1177.00
-
EUR 578.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
2,6-Bis(Trifluoromethyl)Phenol |
abx188185-5g |
Abbexa |
5 g |
EUR 2527 |
|
3,5-Bis(Trifluoromethyl)Phenol |
abx188331-5g |
Abbexa |
5 g |
EUR 314 |
|
Phenol Sulfotransferase (PST) Antibody |
20-abx177976 |
Abbexa |
|
|
|
Phenol Sulfotransferase (PST) Antibody |
20-abx174014 |
Abbexa |
|
|
|
Phenol Sulfotransferase (PST) Antibody |
20-abx174015 |
Abbexa |
|
|
|
Phenol Sulfotransferase (PST) Antibody |
20-abx102281 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1149.00
-
EUR 565.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Phenol Sulfotransferase (PST) Antibody |
20-abx102282 |
Abbexa |
-
EUR 439.00
-
EUR 133.00
-
EUR 1247.00
-
EUR 592.00
-
EUR 328.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
|
Folin & Ciocalteu’s phenol reagent |
abx082175-50ml |
Abbexa |
50 ml |
EUR 189 |
|
The place of the 2 arginine residues intently matches the two-fold screw axis symmetry of the substrate, and this energetically favors the productive enzyme-substrate complicated. Our outcomes point out that the sturdy and particular ligand interactions of Arg251 and Arg394 present a easy proofreading system that controls the step-length throughout consecutive hydrolysis and minimizes lifeless time related to transient, non-productive complexes.